Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 2494..3016 Replicon plasmid pFUJ80154-2
Accession NZ_AP024689
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag FJMB_RS25505 Protein ID WP_000220560.1
Coordinates 2735..3016 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag FJMB_RS25500 Protein ID WP_000121743.1
Coordinates 2494..2745 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS25495 (FJMB80154_p2020) 424..1260 - 837 WP_001050931.1 RepB family plasmid replication initiator protein -
FJMB_RS25500 (FJMB80154_p2030) 2494..2745 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
FJMB_RS25505 (FJMB80154_p2040) 2735..3016 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FJMB_RS25510 3091..3787 + 697 WP_001390273.1 IS1-like element IS1A family transposase -
FJMB_RS25515 3797..4105 + 309 Protein_5 replication initiation protein -
FJMB_RS25520 (FJMB80154_p2080) 5693..6661 + 969 WP_030005799.1 IS5 family transposase -
FJMB_RS25525 (FJMB80154_p2090) 6801..7187 - 387 WP_001039464.1 plasmid stabilization protein StbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS4 - 1..62903 62903


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T39238 WP_000220560.1 NZ_AP024689:2735-3016 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T39238 NZ_AP024689:2735-3016 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT39238 WP_000121743.1 NZ_AP024689:2494-2745 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT39238 NZ_AP024689:2494-2745 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACTACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References