Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4610050..4610547 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FJMB_RS22230 Protein ID WP_001173343.1
Coordinates 4610050..4610223 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag FJMB_RS22235 Protein ID WP_000398619.1
Coordinates 4610251..4610547 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS22220 (4606720) 4606720..4608177 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
FJMB_RS22225 (4608414) 4608414..4609931 + 1518 WP_022646456.1 lysine--tRNA ligase -
FJMB_RS22230 (4610050) 4610050..4610223 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FJMB_RS22235 (4610251) 4610251..4610547 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FJMB_RS22240 (4610776) 4610776..4611048 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
FJMB_RS22245 (4611060) 4611060..4611290 - 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
FJMB_RS22250 (4611471) 4611471..4613102 + 1632 WP_022646455.1 sensor histidine kinase -
FJMB_RS22255 (4613099) 4613099..4613818 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4601196..4610547 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T39231 WP_001173343.1 NZ_AP024687:c4610223-4610050 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T39231 NZ_AP024687:c4610223-4610050 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGCGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT39231 WP_000398619.1 NZ_AP024687:c4610547-4610251 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT39231 NZ_AP024687:c4610547-4610251 [Escherichia coli]
ATGGAAGGTAAAAATAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References