Detailed information of TA system TA039230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4508322..4508917
• Replicon: chromosome
• Accession: NZ_AP024687
• Organism: Escherichia coli strain FUJ80154

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: FJMB_RS21720
• Protein ID: WP_000239581.1
• Coordinates: 4508322..4508672 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: FJMB_RS21725
• Protein ID: WP_001223213.1
• Coordinates: 4508666..4508917 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJMB_RS21700 (4503375)	4503375..4504877	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
FJMB_RS21705 (4505084)	4505084..4506430	+	1347	WP_000483767.1	IS4-like element IS4 family transposase	-
FJMB_RS21710 (4506447)	4506447..4507403	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
FJMB_RS21715 (4507713)	4507713..4508243	+	531	WP_000055075.1	inorganic diphosphatase	-
FJMB_RS21720 (4508322)	4508322..4508672	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
FJMB_RS21725 (4508666)	4508666..4508917	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
FJMB_RS21730 (4509129)	4509129..4509470	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
FJMB_RS21735 (4509473)	4509473..4513252	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4505102..4506430	1328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T39230 WP_000239581.1 NZ_AP024687:c4508672-4508322 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T39230 NZ_AP024687:c4508672-4508322 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT39230 WP_001223213.1 NZ_AP024687:c4508917-4508666 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT39230 NZ_AP024687:c4508917-4508666 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7