Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4205752..4206302 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag FJMB_RS20255 Protein ID WP_001160966.1
Coordinates 4205752..4206066 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag FJMB_RS20260 Protein ID WP_000125568.1
Coordinates 4206069..4206302 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS20230 (4201427) 4201427..4202713 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
FJMB_RS20235 (4202713) 4202713..4203702 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
FJMB_RS20240 (4203699) 4203699..4204520 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
FJMB_RS20245 (4204523) 4204523..4204900 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
FJMB_RS20250 (4204907) 4204907..4205755 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
FJMB_RS20255 (4205752) 4205752..4206066 - 315 WP_001160966.1 CcdB family protein Toxin
FJMB_RS20260 (4206069) 4206069..4206302 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
FJMB_RS20265 (4206388) 4206388..4206867 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
FJMB_RS20270 (4207059) 4207059..4208921 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
FJMB_RS20275 (4208914) 4208914..4209444 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
FJMB_RS20280 (4209552) 4209552..4210883 - 1332 WP_001183201.1 MFS transporter -
FJMB_RS20285 (4210941) 4210941..4211228 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T39224 WP_001160966.1 NZ_AP024687:c4206066-4205752 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T39224 NZ_AP024687:c4206066-4205752 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAATC
CAATCCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCACACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT39224 WP_000125568.1 NZ_AP024687:c4206302-4206069 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT39224 NZ_AP024687:c4206302-4206069 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References