Detailed information of TA system TA039220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3344894..3345599
• Replicon: chromosome
• Accession: NZ_AP024687
• Organism: Escherichia coli strain FUJ80154

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: FJMB_RS16090
• Protein ID: WP_000539521.1
• Coordinates: 3344894..3345280 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: FJMB_RS16095
• Protein ID: WP_001280945.1
• Coordinates: 3345270..3345599 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FJMB_RS16070 (3340898)	3340898..3341524	+	627	WP_001295292.1	glutathione S-transferase GstB	-
FJMB_RS16075 (3341521)	3341521..3342636	-	1116	WP_000554964.1	aldose sugar dehydrogenase YliI	-
FJMB_RS16080 (3342747)	3342747..3343130	-	384	WP_000497137.1	biofilm formation regulator BssR	-
FJMB_RS16085 (3343343)	3343343..3344668	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
FJMB_RS16090 (3344894)	3344894..3345280	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FJMB_RS16095 (3345270)	3345270..3345599	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
FJMB_RS16100 (3345669)	3345669..3346997	-	1329	WP_022645414.1	GGDEF domain-containing protein	-
FJMB_RS16105 (3347005)	3347005..3349353	-	2349	WP_022645413.1	EAL domain-containing protein	-
FJMB_RS16110 (3349530)	3349530..3350441	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T39220 WP_000539521.1 NZ_AP024687:3344894-3345280 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T39220 NZ_AP024687:3344894-3345280 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT39220 WP_001280945.1 NZ_AP024687:3345270-3345599 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT39220 NZ_AP024687:3345270-3345599 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6