Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3042039..3042823 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag FJMB_RS14585 Protein ID WP_000613626.1
Coordinates 3042329..3042823 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag FJMB_RS14580 Protein ID WP_024946541.1
Coordinates 3042039..3042332 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS14570 (3037200) 3037200..3038159 - 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
FJMB_RS14575 (3038732) 3038732..3041905 + 3174 WP_022645526.1 ribonuclease E -
FJMB_RS14580 (3042039) 3042039..3042332 + 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
FJMB_RS14585 (3042329) 3042329..3042823 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
FJMB_RS14590 (3042918) 3042918..3043871 - 954 WP_022645524.1 flagellar hook-associated protein FlgL -
FJMB_RS14595 (3043883) 3043883..3045526 - 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
FJMB_RS14600 (3045592) 3045592..3046533 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
FJMB_RS14605 (3046533) 3046533..3047630 - 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T39219 WP_000613626.1 NZ_AP024687:3042329-3042823 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T39219 NZ_AP024687:3042329-3042823 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT39219 WP_024946541.1 NZ_AP024687:3042039-3042332 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT39219 NZ_AP024687:3042039-3042332 [Escherichia coli]
ATGGCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATAGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCTCCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References