Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2647371..2648009 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag FJMB_RS12575 Protein ID WP_000813794.1
Coordinates 2647833..2648009 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag FJMB_RS12570 Protein ID WP_001270286.1
Coordinates 2647371..2647787 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS12550 (2642523) 2642523..2643464 - 942 WP_022645667.1 ABC transporter permease -
FJMB_RS12555 (2643465) 2643465..2644478 - 1014 WP_022645666.1 ABC transporter ATP-binding protein -
FJMB_RS12560 (2644496) 2644496..2645641 - 1146 WP_000047466.1 ABC transporter substrate-binding protein -
FJMB_RS12565 (2645886) 2645886..2647292 - 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
FJMB_RS12570 (2647371) 2647371..2647787 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
FJMB_RS12575 (2647833) 2647833..2648009 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
FJMB_RS12580 (2648231) 2648231..2648461 + 231 WP_000491567.1 DUF2554 family protein -
FJMB_RS12585 (2648553) 2648553..2650514 - 1962 WP_022645664.1 23S rRNA 5-hydroxycytidine C2501 synthase -
FJMB_RS12590 (2650587) 2650587..2651123 - 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
FJMB_RS12595 (2651215) 2651215..2652387 + 1173 WP_022645663.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T39218 WP_000813794.1 NZ_AP024687:c2648009-2647833 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T39218 NZ_AP024687:c2648009-2647833 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT39218 WP_001270286.1 NZ_AP024687:c2647787-2647371 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT39218 NZ_AP024687:c2647787-2647371 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References