Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 999033..999687 Replicon chromosome
Accession NZ_AP024687
Organism Escherichia coli strain FUJ80154

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag FJMB_RS04790 Protein ID WP_000244781.1
Coordinates 999280..999687 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FJMB_RS04785 Protein ID WP_000354046.1
Coordinates 999033..999299 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJMB_RS04765 (995111) 995111..996544 - 1434 WP_022646090.1 6-phospho-beta-glucosidase BglA -
FJMB_RS04770 (996589) 996589..996900 + 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
FJMB_RS04775 (997064) 997064..997723 + 660 WP_000250269.1 hemolysin III family protein -
FJMB_RS04780 (997800) 997800..998780 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
FJMB_RS04785 (999033) 999033..999299 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FJMB_RS04790 (999280) 999280..999687 + 408 WP_000244781.1 protein YgfX Toxin
FJMB_RS04795 (999727) 999727..1000248 - 522 WP_001055874.1 flavodoxin FldB -
FJMB_RS04800 (1000360) 1000360..1001256 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FJMB_RS04805 (1001281) 1001281..1001991 + 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FJMB_RS04810 (1001997) 1001997..1003730 + 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T39208 WP_000244781.1 NZ_AP024687:999280-999687 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T39208 NZ_AP024687:999280-999687 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT39208 WP_000354046.1 NZ_AP024687:999033-999299 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT39208 NZ_AP024687:999033-999299 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References