Detailed information of TA system TA039198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3144989..3145676
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: K5645_RS14870
• Protein ID: WP_003414624.1
• Coordinates: 3145233..3145676 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: K5645_RS14865
• Protein ID: WP_003414620.1
• Coordinates: 3144989..3145246 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS14845 (MTR2092_29510)	3140309..3141163	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
K5645_RS14850 (MTR2092_29520)	3141219..3142382	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
K5645_RS14855 (MTR2092_29530)	3142399..3143613	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
K5645_RS14860 (MTR2092_29540)	3143621..3144862	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
K5645_RS14865 (MTR2092_29550)	3144989..3145246	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
K5645_RS14870 (MTR2092_29560)	3145233..3145676	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5645_RS14875 (MTR2092_29570)	3145756..3146418	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
K5645_RS14880 (MTR2092_29580)	3146514..3146705	+	192	WP_003414632.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
K5645_RS14885 (MTR2092_29590)	3147037..3148785	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
K5645_RS14890 (MTR2092_29600)	3148881..3149462	+	582	WP_003414644.1	fasciclin domain-containing protein	-
K5645_RS14895 (MTR2092_29610)	3149562..3149828	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T39198 WP_003414624.1 NZ_AP024671:3145233-3145676 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T39198 NZ_AP024671:3145233-3145676 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT39198 WP_003414620.1 NZ_AP024671:3144989-3145246 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT39198 NZ_AP024671:3144989-3145246 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• PDB: 7VWO
• AlphaFold DB: G0TFH0