Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3076915..3077485 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag K5645_RS14510 Protein ID WP_003414166.1
Coordinates 3076915..3077271 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag K5645_RS14515 Protein ID WP_003901465.1
Coordinates 3077255..3077485 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS14490 (MTR2092_28780) 3072367..3074055 - 1689 WP_221062948.1 alpha/beta hydrolase family protein -
K5645_RS14495 (MTR2092_28790) 3074059..3074385 - 327 WP_003414157.1 hypothetical protein -
K5645_RS14500 (MTR2092_28800) 3074558..3075145 + 588 WP_003914429.1 DUF3558 family protein -
K5645_RS14505 (MTR2092_28810) 3075164..3076813 + 1650 WP_003899485.1 CocE/NonD family hydrolase -
K5645_RS14510 (MTR2092_28820) 3076915..3077271 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
K5645_RS14515 (MTR2092_28830) 3077255..3077485 - 231 WP_003901465.1 type II toxin-antitoxin system antitoxin MazE9 Antitoxin
K5645_RS14520 (MTR2092_28840) 3077528..3078571 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
K5645_RS20705 (MTR2092_28850) 3078570..3079037 + 468 WP_003414177.1 DUF1778 domain-containing protein -
K5645_RS14530 (MTR2092_28860) 3079213..3079467 - 255 WP_003917684.1 hypothetical protein -
K5645_RS14535 (MTR2092_28880) 3079615..3080019 + 405 WP_003414181.1 hypothetical protein -
K5645_RS14540 (MTR2092_28890) 3080016..3080207 + 192 WP_003414184.1 hypothetical protein -
K5645_RS20710 3080424..3080684 + 261 Protein_2904 transposase -
K5645_RS14550 3081794..3082051 + 258 WP_003899489.1 hypothetical protein -
K5645_RS14555 (MTR2092_28910) 3082156..3082467 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3070005..3098450 28445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T39194 WP_003414166.1 NZ_AP024671:c3077271-3076915 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T39194 NZ_AP024671:c3077271-3076915 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT39194 WP_003901465.1 NZ_AP024671:c3077485-3077255 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT39194 NZ_AP024671:c3077485-3077255 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References