Detailed information of TA system TA039186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2822133..2822773
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: K5645_RS13100
• Protein ID: WP_003412970.1
• Coordinates: 2822133..2822552 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: K5645_RS13105
• Protein ID: WP_003412975.1
• Coordinates: 2822549..2822773 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS13070 (MTR2092_26050)	2817723..2818445	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
K5645_RS13075 (MTR2092_26060)	2818962..2819184	+	223	Protein_2611	type II toxin-antitoxin system VapB family antitoxin	-
K5645_RS13080 (MTR2092_26070)	2819181..2819582	+	402	WP_003412963.1	PIN domain-containing protein	-
K5645_RS13085 (MTR2092_26080)	2819617..2820537	-	921	WP_003412965.1	restriction endonuclease	-
K5645_RS13090 (MTR2092_26090)	2820878..2821123	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
K5645_RS13095 (MTR2092_26100)	2821182..2822132	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
K5645_RS13100 (MTR2092_26110)	2822133..2822552	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5645_RS13105 (MTR2092_26120)	2822549..2822773	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
K5645_RS13110 (MTR2092_26130)	2822804..2825647	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
K5645_RS13115 (MTR2092_26140)	2825719..2826120	-	402	WP_003412981.1	hypothetical protein	-
K5645_RS13120 (MTR2092_26150)	2826120..2826590	-	471	WP_003899364.1	transcription antitermination factor NusB	-
K5645_RS13125 (MTR2092_26160)	2826593..2827156	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T39186 WP_003412970.1 NZ_AP024671:c2822552-2822133 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T39186 NZ_AP024671:c2822552-2822133 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT39186 WP_003412975.1 NZ_AP024671:c2822773-2822549 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT39186 NZ_AP024671:c2822773-2822549 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09