Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2345134..2345829 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name vapC Uniprot ID O53501
Locus tag K5645_RS10840 Protein ID WP_003410811.1
Coordinates 2345134..2345568 (-) Length 145 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TMN0
Locus tag K5645_RS10845 Protein ID WP_003410814.1
Coordinates 2345575..2345829 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS10830 (MTR2092_21770) 2341288..2344329 + 3042 WP_003899171.1 DEAD/DEAH box helicase -
K5645_RS10835 (MTR2092_21780) 2344322..2345155 + 834 WP_003899172.1 SWIM zinc finger family protein -
K5645_RS10840 (MTR2092_21790) 2345134..2345568 - 435 WP_003410811.1 type II toxin-antitoxin system VapC family toxin Toxin
K5645_RS10845 (MTR2092_21800) 2345575..2345829 - 255 WP_003410814.1 antitoxin Antitoxin
K5645_RS10850 2345845..2346102 - 258 WP_003410816.1 hypothetical protein -
K5645_RS10855 (MTR2092_21810) 2347049..2347345 + 297 WP_003410820.1 PE family protein -
K5645_RS10860 (MTR2092_21820) 2347401..2348132 + 732 WP_003900467.1 PPE family protein -
K5645_RS10865 (MTR2092_21830) 2348672..2349418 - 747 WP_003901330.1 proteasome subunit alpha -
K5645_RS10870 (MTR2092_21840) 2349415..2350290 - 876 WP_003411023.1 proteasome subunit beta -
K5645_RS10875 (MTR2092_21850) 2350287..2350481 - 195 WP_003411026.1 ubiquitin-like protein Pup -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 145 a.a.        Molecular weight: 15662.08 Da        Isoelectric Point: 7.4681

>T39183 WP_003410811.1 NZ_AP024671:c2345568-2345134 [Mycobacterium tuberculosis]
MKIVDANVLLYAVNTTSEHHKPSLRWLDGALSGADRVGFAWVPLLAFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFEGVRWDQPPALL

Download         Length: 435 bp

>T39183 NZ_AP024671:c2345568-2345134 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9417.57 Da        Isoelectric Point: 8.9912

>AT39183 WP_003410814.1 NZ_AP024671:c2345829-2345575 [Mycobacterium tuberculosis]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Download         Length: 255 bp

>AT39183 NZ_AP024671:c2345829-2345575 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FB09

Antitoxin

Source ID Structure
AlphaFold DB G0TMN0

References