Detailed information of TA system TA039180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2232662..2233288
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64926
• Locus tag: K5645_RS10355
• Protein ID: WP_003410075.1
• Coordinates: 2232890..2233288 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A806JLL8
• Locus tag: K5645_RS10350
• Protein ID: WP_003911750.1
• Coordinates: 2232662..2232889 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS10340 (MTR2092_20660)	2230701..2231045	-	345	WP_003410065.1	ferredoxin family protein	-
K5645_RS10345 (MTR2092_20670)	2231234..2232403	-	1170	WP_003899126.1	ATP-binding protein	-
K5645_RS10350 (MTR2092_20680)	2232662..2232889	+	228	WP_003911750.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K5645_RS10355 (MTR2092_20690)	2232890..2233288	+	399	WP_003410075.1	PIN domain nuclease	Toxin
K5645_RS10360 (MTR2092_20700)	2233471..2233902	-	432	WP_003410078.1	MarR family winged helix-turn-helix transcriptional regulator	-
K5645_RS10365 (MTR2092_20710)	2234003..2234437	+	435	WP_003914358.1	DUF1398 domain-containing protein	-
K5645_RS10370	2234874..2235053	-	180	Protein_2073	hypothetical protein	-
K5645_RS10375 (MTR2092_20720)	2235141..2235761	+	621	WP_003410086.1	IS110 family transposase	-
K5645_RS10380 (MTR2092_20730)	2235715..2236305	+	591	WP_003899131.1	IS110 family transposase	-
K5645_RS10385 (MTR2092_20740)	2236433..2237689	-	1257	WP_003902247.1	HNH endonuclease signature motif containing protein	-
K5645_RS10390 (MTR2092_20750)	2238043..2238243	+	201	Protein_2077	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-120)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-43)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14731.02 Da
• Isoelectric Point: 6.7067

>T39180 WP_003410075.1 NZ_AP024671:2232890-2233288 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Nucleotide

• Length: 399 bp

>T39180 NZ_AP024671:2232890-2233288 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8346.16 Da
• Isoelectric Point: 4.1621

>AT39180 WP_003911750.1 NZ_AP024671:2232662-2232889 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Nucleotide

• Length: 228 bp

>AT39180 NZ_AP024671:2232662-2232889 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Structures

Toxin

• PDB: 4CHG

Antitoxin

• AlphaFold DB: A0A806JLL8