Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2200031..2200719 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag K5645_RS10185 Protein ID WP_003409958.1
Coordinates 2200031..2200450 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag K5645_RS10190 Protein ID WP_003409968.1
Coordinates 2200459..2200719 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS10165 (MTR2092_20300) 2195526..2196374 + 849 WP_003901306.1 class I SAM-dependent methyltransferase -
K5645_RS10170 (MTR2092_20310) 2196337..2197782 - 1446 WP_003899115.1 APC family permease -
K5645_RS10175 (MTR2092_20320) 2197961..2198647 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
K5645_RS10180 (MTR2092_20330) 2198838..2199806 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
K5645_RS10185 (MTR2092_20340) 2200031..2200450 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
K5645_RS10190 (MTR2092_20350) 2200459..2200719 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5645_RS10195 (MTR2092_20360) 2200862..2202538 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
K5645_RS10200 (MTR2092_20370) 2202526..2203179 - 654 WP_003409976.1 cutinase Cfp21 -
K5645_RS10205 (MTR2092_20380) 2203294..2203524 + 231 WP_003409980.1 DUF167 domain-containing protein -
K5645_RS10210 (MTR2092_20390) 2203609..2204520 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
K5645_RS10215 (MTR2092_20400) 2204629..2205228 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T39177 WP_003409958.1 NZ_AP024671:c2200450-2200031 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T39177 NZ_AP024671:c2200450-2200031 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT39177 WP_003409968.1 NZ_AP024671:c2200719-2200459 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT39177 NZ_AP024671:c2200719-2200459 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References