Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1750676..1751289 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag K5645_RS08105 Protein ID WP_003407786.1
Coordinates 1750885..1751289 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag K5645_RS08100 Protein ID WP_009935474.1
Coordinates 1750676..1750879 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS08070 (MTR2092_16030) 1746081..1746461 + 381 WP_003407768.1 fumarate reductase subunit C -
K5645_RS08075 (MTR2092_16040) 1746458..1746835 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
K5645_RS08080 (MTR2092_16050) 1746903..1747511 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
K5645_RS08085 (MTR2092_16060) 1747689..1748843 + 1155 Protein_1616 MMPL family transporter -
K5645_RS08090 (MTR2092_16070) 1748853..1749299 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
K5645_RS08095 (MTR2092_16080) 1749334..1750623 + 1290 WP_003407781.1 threonine ammonia-lyase -
K5645_RS08100 (MTR2092_16090) 1750676..1750879 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5645_RS08105 (MTR2092_16100) 1750885..1751289 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
K5645_RS08110 (MTR2092_16110) 1751306..1753048 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
K5645_RS08115 (MTR2092_16120) 1753041..1755338 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T39171 WP_003407786.1 NZ_AP024671:1750885-1751289 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T39171 NZ_AP024671:1750885-1751289 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT39171 WP_009935474.1 NZ_AP024671:1750676-1750879 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT39171 NZ_AP024671:1750676-1750879 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References