Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1672178..1672794 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag K5645_RS07760 Protein ID WP_003407593.1
Coordinates 1672477..1672794 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag K5645_RS07755 Protein ID WP_003900349.1
Coordinates 1672178..1672480 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS07745 (MTR2092_15370) 1668064..1669911 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
K5645_RS07750 (MTR2092_15380) 1669912..1672164 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
K5645_RS07755 (MTR2092_15390) 1672178..1672480 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
K5645_RS07760 (MTR2092_15400) 1672477..1672794 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
K5645_RS07765 (MTR2092_15410) 1672791..1673795 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
K5645_RS07770 (MTR2092_15420) 1673848..1675137 + 1290 WP_003902090.1 serine hydrolase domain-containing protein -
K5645_RS07775 (MTR2092_15430) 1675210..1675935 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
K5645_RS07780 (MTR2092_15440) 1676041..1676253 - 213 WP_003898900.1 dodecin family protein -
K5645_RS20580 (MTR2092_15450) 1676314..1676712 + 399 WP_003900351.1 hypothetical protein -
K5645_RS07790 (MTR2092_15460) 1676757..1677785 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T39170 WP_003407593.1 NZ_AP024671:1672477-1672794 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T39170 NZ_AP024671:1672477-1672794 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT39170 WP_003900349.1 NZ_AP024671:1672178-1672480 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT39170 NZ_AP024671:1672178-1672480 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References