Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1227161..1227792 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag K5645_RS05780 Protein ID WP_003405820.1
Coordinates 1227161..1227472 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag K5645_RS05785 Protein ID WP_003405836.1
Coordinates 1227472..1227792 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS05760 (MTR2092_11360) 1222642..1224066 - 1425 WP_003405805.1 class II fumarate hydratase -
K5645_RS05765 (MTR2092_11370) 1224097..1225185 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
K5645_RS05770 (MTR2092_11380) 1225241..1225885 + 645 WP_003915494.1 DUF4245 domain-containing protein -
K5645_RS05775 (MTR2092_11390) 1225892..1227049 - 1158 WP_221063014.1 AI-2E family transporter -
K5645_RS05780 (MTR2092_11400) 1227161..1227472 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
K5645_RS05785 (MTR2092_11410) 1227472..1227792 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
K5645_RS05790 1227802..1229338 + 1537 Protein_1157 carboxylesterase/lipase family protein -
K5645_RS05795 (MTR2092_11450) 1229345..1230457 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
K5645_RS05800 (MTR2092_11460) 1230467..1230724 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
K5645_RS05805 (MTR2092_11470) 1230714..1231961 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
K5645_RS05810 (MTR2092_11480) 1231958..1232596 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T39166 WP_003405820.1 NZ_AP024671:c1227472-1227161 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T39166 NZ_AP024671:c1227472-1227161 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT39166 WP_003405836.1 NZ_AP024671:c1227792-1227472 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT39166 NZ_AP024671:c1227792-1227472 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References