Detailed information of TA system TA039164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1010982..1011601
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: K5645_RS04750
• Protein ID: WP_003404726.1
• Coordinates: 1011167..1011601 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: K5645_RS04745
• Protein ID: WP_003898641.1
• Coordinates: 1010982..1011161 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS04730 (MTR2092_09310)	1006455..1008035	+	1581	WP_003909313.1	serine hydrolase	-
K5645_RS04735 (MTR2092_09320)	1008032..1010425	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
K5645_RS04740 (MTR2092_09340)	1010698..1010856	+	159	WP_003404720.1	hypothetical protein	-
K5645_RS04745 (MTR2092_09350)	1010982..1011161	+	180	WP_003898641.1	antitoxin	Antitoxin
K5645_RS04750 (MTR2092_09360)	1011167..1011601	+	435	WP_003404726.1	SRPBCC family protein	Toxin
K5645_RS04755 (MTR2092_09370)	1011699..1012472	+	774	WP_003404735.1	VOC family protein	-
K5645_RS04760 (MTR2092_09380)	1012537..1012986	+	450	WP_003404738.1	hypothetical protein	-
K5645_RS04765 (MTR2092_09390)	1013121..1013351	-	231	WP_003898642.1	hypothetical protein	-
K5645_RS04770 (MTR2092_09400)	1013518..1015026	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
K5645_RS04775 (MTR2092_09410)	1015028..1016266	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T39164 WP_003404726.1 NZ_AP024671:1011167-1011601 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T39164 NZ_AP024671:1011167-1011601 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT39164 WP_003898641.1 NZ_AP024671:1010982-1011161 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT39164 NZ_AP024671:1010982-1011161 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5