Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 750617..751250 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag K5645_RS03425 Protein ID WP_003403365.1
Coordinates 750617..751000 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag K5645_RS03430 Protein ID WP_003403368.1
Coordinates 751095..751250 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS03400 (MTR2092_06720) 745909..746445 + 537 WP_003403341.1 50S ribosomal protein L10 -
K5645_RS03405 (MTR2092_06730) 746482..746874 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
K5645_RS03410 (MTR2092_06740) 746867..747562 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
K5645_RS03415 (MTR2092_06750) 747633..749138 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
K5645_RS03420 (MTR2092_06760) 749219..750229 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
K5645_RS03425 (MTR2092_06770) 750617..751000 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
K5645_RS03430 (MTR2092_06780) 751095..751250 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5645_RS03435 (MTR2092_06790) 751326..752042 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
K5645_RS03440 (MTR2092_06800) 752318..752626 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
K5645_RS03445 (MTR2092_06810) 752613..752858 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
K5645_RS03450 (MTR2092_06820) 752968..753405 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
K5645_RS03455 (MTR2092_06830) 753402..753656 - 255 WP_003911263.1 antitoxin VapB7 -
K5645_RS03460 (MTR2092_06840) 753770..756133 + 2364 WP_003901895.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T39159 WP_003403365.1 NZ_AP024671:c751000-750617 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T39159 NZ_AP024671:c751000-750617 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT39159 WP_003403368.1 NZ_AP024671:c751250-751095 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT39159 NZ_AP024671:c751250-751095 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References