Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 713510..714159 Replicon chromosome
Accession NZ_AP024671
Organism Mycobacterium tuberculosis strain R2092

Toxin (Protein)


Gene name vapC Uniprot ID P67241
Locus tag K5645_RS03240 Protein ID WP_003403236.1
Coordinates 713764..714159 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ34
Locus tag K5645_RS03235 Protein ID WP_003403235.1
Coordinates 713510..713764 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5645_RS20420 708636..708719 + 84 Protein_641 galactose-1-phosphate uridylyltransferase -
K5645_RS20425 708738..709819 + 1082 Protein_642 galactose-1-phosphate uridylyltransferase -
K5645_RS03220 (MTR2092_06380) 709816..710907 + 1092 WP_003900977.1 galactokinase -
K5645_RS03225 (MTR2092_06400) 711302..712366 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
K5645_RS03230 (MTR2092_06410) 712470..713417 + 948 WP_003403232.1 DUF732 domain-containing protein -
K5645_RS03235 (MTR2092_06420) 713510..713764 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 Antitoxin
K5645_RS03240 (MTR2092_06430) 713764..714159 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 Toxin
K5645_RS03245 (MTR2092_06440) 714253..714993 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
K5645_RS03250 (MTR2092_06450) 715125..715385 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
K5645_RS03255 (MTR2092_06460) 715382..715789 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin -
K5645_RS03260 (MTR2092_06470) 715861..717012 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
K5645_RS03265 (MTR2092_06480) 717105..718832 - 1728 WP_003901883.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-83)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14250.04 Da        Isoelectric Point: 4.7475

>T39157 WP_003403236.1 NZ_AP024671:713764-714159 [Mycobacterium tuberculosis]
MVIDTSALVAMLSDEPDAERFEAAVEADHIRLMSTASYLETALVIEARFGEPGGRELDLWLHRAAVDLVAVHADQADAAR
AAYRTYGKGRHRAGLNYGDCFSYGLAKISGQPLLFKGEDFQHTDIATVALP

Download         Length: 396 bp

>T39157 NZ_AP024671:713764-714159 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9139.52 Da        Isoelectric Point: 9.2702

>AT39157 WP_003403235.1 NZ_AP024671:713510-713764 [Mycobacterium tuberculosis]
MALSIKHPEADRLARALAARTGETLTEAVVTALRERLARETGRARVVPLRDELAAIRHRCAALPVVDNRSAEAILGYDER
GLPA

Download         Length: 255 bp

>AT39157 NZ_AP024671:713510-713764 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4XGR
PDB 4XGQ

Antitoxin

Source ID Structure
PDB 4XGR
PDB 4XGQ
AlphaFold DB A0A7U4BSC2

References