Detailed information of TA system TA039154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 694254..694900
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: K5645_RS03100
• Protein ID: WP_003403137.1
• Coordinates: 694254..694667 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: K5645_RS03105
• Protein ID: WP_003403139.1
• Coordinates: 694664..694900 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS03080 (MTR2092_06100)	690337..691887	+	1551	WP_003403119.1	MlaD family protein	-
K5645_RS03085 (MTR2092_06110)	691939..692331	-	393	WP_221063038.1	type II toxin-antitoxin system toxin ribonuclease C4	-
K5645_RS03090 (MTR2092_06120)	692328..692585	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
K5645_RS03095 (MTR2092_06130)	692768..694003	-	1236	WP_003403128.1	ATP-binding protein	-
K5645_RS03100 (MTR2092_06150)	694254..694667	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5645_RS03105 (MTR2092_06160)	694664..694900	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	Antitoxin
K5645_RS03110 (MTR2092_06170)	695004..695510	-	507	WP_003900973.1	two component system sensor kinase HK1	-
K5645_RS03115 (MTR2092_06180)	695624..696154	-	531	WP_003917320.1	two component system sensor kinase HK2	-
K5645_RS03120 (MTR2092_06190)	696138..696833	-	696	WP_003918099.1	two-component system response regulator TcrA	-
K5645_RS03125 (MTR2092_06200)	696956..697267	+	312	WP_003403164.1	hypothetical protein	-
K5645_RS03130 (MTR2092_06210)	697339..698289	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
K5645_RS03135 (MTR2092_06220)	698530..699114	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
K5645_RS03140 (MTR2092_06230)	699116..699826	+	711	Protein_627	IS607 family element RNA-guided endonuclease TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T39154 WP_003403137.1 NZ_AP024671:c694667-694254 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T39154 NZ_AP024671:c694667-694254 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT39154 WP_003403139.1 NZ_AP024671:c694900-694664 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT39154 NZ_AP024671:c694900-694664 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89