Detailed information of TA system TA039153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 691939..692585
• Replicon: chromosome
• Accession: NZ_AP024671
• Organism: Mycobacterium tuberculosis strain R2092

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K5645_RS03085
• Protein ID: WP_221063038.1
• Coordinates: 691939..692331 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ86
• Locus tag: K5645_RS03090
• Protein ID: WP_003403125.1
• Coordinates: 692328..692585 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5645_RS03070 (MTR2092_06080)	687601..689127	+	1527	WP_003403112.1	virulence factor Mce family protein	-
K5645_RS03075 (MTR2092_06090)	689190..690332	+	1143	WP_221062995.1	virulence factor Mce family protein	-
K5645_RS03080 (MTR2092_06100)	690337..691887	+	1551	WP_003403119.1	MlaD family protein	-
K5645_RS03085 (MTR2092_06110)	691939..692331	-	393	WP_221063038.1	type II toxin-antitoxin system toxin ribonuclease C4	Toxin
K5645_RS03090 (MTR2092_06120)	692328..692585	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	Antitoxin
K5645_RS03095 (MTR2092_06130)	692768..694003	-	1236	WP_003403128.1	ATP-binding protein	-
K5645_RS03100 (MTR2092_06150)	694254..694667	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	-
K5645_RS03105 (MTR2092_06160)	694664..694900	-	237	WP_003403139.1	type II toxin-antitoxin system antitoxin VapB27	-
K5645_RS03110 (MTR2092_06170)	695004..695510	-	507	WP_003900973.1	two component system sensor kinase HK1	-
K5645_RS03115 (MTR2092_06180)	695624..696154	-	531	WP_003917320.1	two component system sensor kinase HK2	-
K5645_RS03120 (MTR2092_06190)	696138..696833	-	696	WP_003918099.1	two-component system response regulator TcrA	-
K5645_RS03125 (MTR2092_06200)	696956..697267	+	312	WP_003403164.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14098.11 Da
• Isoelectric Point: 4.6558

>T39153 WP_221063038.1 NZ_AP024671:c692331-691939 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVMTI

Nucleotide

• Length: 393 bp

>T39153 NZ_AP024671:c692331-691939 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATGACGATCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9681.06 Da
• Isoelectric Point: 7.0175

>AT39153 WP_003403125.1 NZ_AP024671:c692585-692328 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Nucleotide

• Length: 258 bp

>AT39153 NZ_AP024671:c692585-692328 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Structures

Antitoxin

• AlphaFold DB: G0TQ86