Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 512363..512999 | Replicon | chromosome |
| Accession | NZ_AP024627 | ||
| Organism | Bacillus subtilis strain BEST3136 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | K6J86_RS02590 | Protein ID | WP_003156187.1 |
| Coordinates | 512649..512999 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | K6J86_RS02585 | Protein ID | WP_003225183.1 |
| Coordinates | 512363..512644 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K6J86_RS02565 (508722) | 508722..509321 | - | 600 | WP_003246687.1 | rhomboid family intramembrane serine protease | - |
| K6J86_RS02570 (509416) | 509416..509781 | + | 366 | WP_003234281.1 | holo-ACP synthase | - |
| K6J86_RS02575 (509947) | 509947..510963 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| K6J86_RS02580 (511078) | 511078..512247 | + | 1170 | WP_003234284.1 | alanine racemase | - |
| K6J86_RS02585 (512363) | 512363..512644 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| K6J86_RS02590 (512649) | 512649..512999 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| K6J86_RS02595 (513114) | 513114..513938 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| K6J86_RS02600 (513943) | 513943..514308 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| K6J86_RS02605 (514312) | 514312..514713 | + | 402 | WP_003246640.1 | serine/threonine-protein kinase RsbT | - |
| K6J86_RS02610 (514725) | 514725..515732 | + | 1008 | WP_003234295.1 | phosphoserine phosphatase RsbU | - |
| K6J86_RS02615 (515794) | 515794..516123 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| K6J86_RS02620 (516120) | 516120..516602 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| K6J86_RS02625 (516568) | 516568..517356 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| K6J86_RS02630 (517356) | 517356..517955 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T39099 WP_003156187.1 NZ_AP024627:512649-512999 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T39099 NZ_AP024627:512649-512999 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT39099 WP_003225183.1 NZ_AP024627:512363-512644 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT39099 NZ_AP024627:512363-512644 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|