39008

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1317012..1317928 Replicon chromosome
Accession NZ_AP024623
Organism Bacillus subtilis strain BEST3102

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag K6J99_RS06885 Protein ID WP_003244695.1
Coordinates 1317182..1317928 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag K6J99_RS06880 Protein ID WP_003232646.1
Coordinates 1317012..1317182 (-) Length 57 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J99_RS06845 (1313875) 1313875..1314204 + 330 WP_003232660.1 XkdW family protein -
K6J99_RS06850 (1314201) 1314201..1314365 + 165 WP_003232658.1 XkdX family protein -
K6J99_RS06855 (1314409) 1314409..1315248 + 840 WP_003245597.1 phage-like element PBSX protein XepA -
K6J99_RS06860 (1315301) 1315301..1315570 + 270 WP_003232655.1 hemolysin XhlA family protein -
K6J99_RS06865 (1315583) 1315583..1315846 + 264 WP_003232653.1 phage holin -
K6J99_RS06870 (1315859) 1315859..1316752 + 894 WP_003245230.1 N-acetylmuramoyl-L-alanine amidase -
K6J99_RS06875 (1316789) 1316789..1316926 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
K6J99_RS06880 (1317012) 1317012..1317182 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
K6J99_RS06885 (1317182) 1317182..1317928 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
K6J99_RS06890 (1318038) 1318038..1319039 - 1002 WP_003232642.1 inorganic phosphate transporter -
K6J99_RS06895 (1319052) 1319052..1319669 - 618 WP_003218470.1 DUF47 domain-containing protein -
K6J99_RS06900 (1319945) 1319945..1321261 - 1317 WP_003244819.1 serine/threonine exchanger -
K6J99_RS06905 (1321650) 1321650..1322600 + 951 WP_003245086.1 ring-cleaving dioxygenase -
K6J99_RS06910 (1322701) 1322701..1322847 + 147 WP_003244977.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1283020..1322847 39827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-238)

Antitoxin

(1-56)


Sequences


Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T39008 WP_003244695.1 NZ_AP024623:c1317928-1317182 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

>T39008 NZ_AP024623:c1317928-1317182 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTCCGCCATTCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATATGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT39008 WP_003232646.1 NZ_AP024623:c1317182-1317012 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp

>AT39008 NZ_AP024623:c1317182-1317012 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 3O6Q


Antitoxin

Source ID Structure
AlphaFold DB G4EXD8

References