39007

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 507675..508311 Replicon chromosome
Accession NZ_AP024623
Organism Bacillus subtilis strain BEST3102

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag K6J99_RS02570 Protein ID WP_003156187.1
Coordinates 507961..508311 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag K6J99_RS02565 Protein ID WP_003225183.1
Coordinates 507675..507956 (+) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J99_RS02545 (504034) 504034..504633 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
K6J99_RS02550 (504728) 504728..505093 + 366 WP_003234281.1 holo-ACP synthase -
K6J99_RS02555 (505259) 505259..506275 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
K6J99_RS02560 (506390) 506390..507559 + 1170 WP_003234284.1 alanine racemase -
K6J99_RS02565 (507675) 507675..507956 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
K6J99_RS02570 (507961) 507961..508311 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K6J99_RS02575 (508426) 508426..509250 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
K6J99_RS02580 (509255) 509255..509620 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
K6J99_RS02585 (509624) 509624..510025 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
K6J99_RS02590 (510037) 510037..511044 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
K6J99_RS02595 (511106) 511106..511435 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
K6J99_RS02600 (511432) 511432..511914 + 483 WP_003234299.1 anti-sigma B factor RsbW -
K6J99_RS02605 (511880) 511880..512668 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
K6J99_RS02610 (512668) 512668..513267 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-111)

Antitoxin

(12-45)


Sequences


Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T39007 WP_003156187.1 NZ_AP024623:507961-508311 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T39007 NZ_AP024623:507961-508311 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT39007 WP_003225183.1 NZ_AP024623:507675-507956 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT39007 NZ_AP024623:507675-507956 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8


Antitoxin

Source ID Structure
AlphaFold DB G4NU32

References