Detailed information of TA system TA038961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 518254..518890
• Replicon: chromosome
• Accession: NZ_AP024621
• Organism: Bacillus subtilis strain BEST3095

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K6J89_RS02635
• Protein ID: WP_003156187.1
• Coordinates: 518540..518890 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: K6J89_RS02630
• Protein ID: WP_003225183.1
• Coordinates: 518254..518535 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K6J89_RS02610 (514613)	514613..515212	-	600	WP_003246687.1	rhomboid family intramembrane serine protease	-
K6J89_RS02615 (515307)	515307..515672	+	366	WP_003234281.1	holo-ACP synthase	-
K6J89_RS02620 (515838)	515838..516854	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
K6J89_RS02625 (516969)	516969..518138	+	1170	WP_003234284.1	alanine racemase	-
K6J89_RS02630 (518254)	518254..518535	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K6J89_RS02635 (518540)	518540..518890	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K6J89_RS02640 (519005)	519005..519829	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
K6J89_RS02645 (519834)	519834..520199	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
K6J89_RS02650 (520203)	520203..520604	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
K6J89_RS02655 (520616)	520616..521623	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
K6J89_RS02660 (521685)	521685..522014	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
K6J89_RS02665 (522011)	522011..522493	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
K6J89_RS02670 (522459)	522459..523247	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
K6J89_RS02675 (523247)	523247..523846	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T38961 WP_003156187.1 NZ_AP024621:518540-518890 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T38961 NZ_AP024621:518540-518890 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT38961 WP_003225183.1 NZ_AP024621:518254-518535 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT38961 NZ_AP024621:518254-518535 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• PDB: 4ME7
• AlphaFold DB: G4NU32