Detailed information of TA system TA038904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 93063..93703
• Replicon: chromosome
• Accession: NZ_AP024597
• Organism: Stygiolobus caldivivus strain KN-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KN1_RS00435
• Protein ID: WP_221288673.1
• Coordinates: 93317..93703 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KN1_RS00430
• Protein ID: WP_221290405.1
• Coordinates: 93063..93320 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KN1_RS00405 (KN1_00920)	88795..89157	+	363	WP_221288666.1	ribbon-helix-helix domain-containing protein	-
KN1_RS00410 (KN1_00930)	89133..89507	+	375	WP_221288668.1	clan AA aspartic protease	-
KN1_RS00415 (KN1_00940)	89958..90692	-	735	WP_221288670.1	endonuclease NucS	-
KN1_RS00420 (KN1_00950)	90920..92572	+	1653	WP_221288671.1	DUF87 domain-containing protein	-
KN1_RS00425 (KN1_00960)	92578..92832	+	255	WP_221288672.1	hypothetical protein	-
KN1_RS00430 (KN1_00970)	93063..93320	+	258	WP_221290405.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KN1_RS00435 (KN1_00980)	93317..93703	+	387	WP_221288673.1	type II toxin-antitoxin system VapC family toxin	Toxin
KN1_RS00440 (KN1_00990)	94375..94815	-	441	WP_221288674.1	hypothetical protein	-
KN1_RS00445 (KN1_01000)	95158..95997	+	840	WP_221288675.1	hypothetical protein	-
KN1_RS00450 (KN1_01010)	96382..96789	+	408	WP_221288676.1	rhodanese-like domain-containing protein	-
KN1_RS00455 (KN1_01020)	97410..98399	-	990	WP_221288677.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-120)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14740.12 Da
• Isoelectric Point: 4.6190

>T38904 WP_221288673.1 NZ_AP024597:93317-93703 [Stygiolobus caldivivus]
VIFLDANFLIYLNLGVRGVEEFYYKLLSEENTALDPLVIDEVVYVSKKKYGVKYEDTLEFLDNVVLPYSVVFPITVEEYK
RAKDIMLVGLKPSDAFHVAVMLNNSVLRVVSEDADFDKVKGVQRVWLR

Nucleotide

• Length: 387 bp

>T38904 NZ_AP024597:93317-93703 [Stygiolobus caldivivus]
GTGATTTTCCTCGACGCTAACTTCCTCATCTATTTGAACTTAGGTGTAAGAGGAGTAGAAGAGTTTTATTATAAGCTCCT
CAGTGAGGAAAATACTGCACTGGACCCCTTAGTTATTGATGAAGTAGTCTATGTGTCTAAGAAGAAATACGGTGTGAAGT
ATGAAGACACTTTGGAGTTTTTAGACAATGTAGTCCTCCCGTACTCTGTAGTATTCCCTATAACGGTTGAGGAGTATAAG
AGGGCGAAGGACATAATGCTGGTGGGCTTAAAGCCATCCGACGCTTTCCATGTAGCTGTTATGTTAAATAACTCCGTCTT
AAGGGTCGTCAGTGAAGACGCAGACTTCGATAAAGTGAAGGGTGTCCAAAGGGTTTGGCTTAGATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9640.39 Da
• Isoelectric Point: 8.4281

>AT38904 WP_221290405.1 NZ_AP024597:93063-93320 [Stygiolobus caldivivus]
MALKVEVGKKGYVIIPKSIRDLVGIKEGDVLILRVTDGKIILEPERKVDLESLRKYMEGHKKKISYAKEAKLGELANISL
EEEFE

Nucleotide

• Length: 258 bp

>AT38904 NZ_AP024597:93063-93320 [Stygiolobus caldivivus]
TTGGCCCTGAAGGTGGAAGTCGGGAAAAAAGGTTACGTAATAATCCCTAAAAGTATAAGGGACCTCGTCGGGATTAAAGA
GGGGGACGTACTAATACTACGTGTCACAGACGGGAAAATTATCCTCGAGCCTGAAAGGAAAGTCGACCTCGAGTCCTTGA
GGAAATATATGGAGGGACACAAGAAGAAAATATCGTATGCAAAGGAGGCTAAGTTAGGTGAGCTGGCTAATATAAGCCTA
GAGGAGGAATTTGAGTGA