Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 29204..29940 Replicon plasmid pKLVA-1
Accession NZ_AP024593
Organism Klebsiella variicola strain JCM 12419

Toxin (Protein)


Gene name tacT Uniprot ID A0A6M6A403
Locus tag KI238_RS27005 Protein ID WP_023288256.1
Coordinates 29458..29940 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KI238_RS27000 Protein ID WP_003026799.1
Coordinates 29204..29470 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI238_RS26960 (KLVA_52240) 24485..24703 + 219 WP_119867233.1 hypothetical protein -
KI238_RS26965 (KLVA_52250) 24782..25108 + 327 WP_123797897.1 hypothetical protein -
KI238_RS26970 (KLVA_52260) 25348..25605 + 258 WP_119867235.1 hypothetical protein -
KI238_RS26975 (KLVA_52270) 25605..26171 + 567 WP_119867236.1 hypothetical protein -
KI238_RS26980 (KLVA_52280) 26202..26693 + 492 WP_119867237.1 DNA-binding protein -
KI238_RS26985 (KLVA_52290) 26717..27229 - 513 WP_123797898.1 hypothetical protein -
KI238_RS26990 (KLVA_52300) 27852..28637 + 786 WP_123797899.1 hypothetical protein -
KI238_RS26995 (KLVA_52310) 28800..28997 - 198 WP_123797900.1 hypothetical protein -
KI238_RS27000 (KLVA_52320) 29204..29470 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KI238_RS27005 29458..29940 + 483 WP_023288256.1 GNAT family N-acetyltransferase Toxin
KI238_RS27010 (KLVA_52330) 30253..30771 + 519 Protein_35 AraC family transcriptional regulator -
KI238_RS27015 (KLVA_52340) 30914..31213 - 300 WP_101880805.1 putative quinol monooxygenase -
KI238_RS27020 (KLVA_52350) 32243..33655 - 1413 WP_119867240.1 amino acid permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..163252 163252
- flank IS/Tn - - 31540..31641 101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17296.98 Da        Isoelectric Point: 8.7400

>T38902 WP_023288256.1 NZ_AP024593:29458-29940 [Klebsiella variicola]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDISLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYVHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T38902 NZ_AP024593:29458-29940 [Klebsiella variicola]
GTGGGATGTATAACAGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGAGAAACGGTACTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAGGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACAGGTACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATATCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGCTTCTATGTTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT38902 WP_003026799.1 NZ_AP024593:29204-29470 [Klebsiella variicola]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT38902 NZ_AP024593:29204-29470 [Klebsiella variicola]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6M6A403

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References