Detailed information of TA system TA038900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21023..21666
• Replicon: plasmid pKLVA-1
• Accession: NZ_AP024593
• Organism: Klebsiella variicola strain JCM 12419

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: KI238_RS26940
• Protein ID: WP_119867229.1
• Coordinates: 21250..21666 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: KI238_RS26935
• Protein ID: WP_119867228.1
• Coordinates: 21023..21253 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI238_RS26915 (KLVA_52140)	16391..17533	-	1143	WP_119867224.1	toll/interleukin-1 receptor domain-containing protein	-
KI238_RS26920 (KLVA_52150)	17667..19283	-	1617	WP_119867225.1	CBASS cGAMP-activated phospholipase	-
KI238_RS26925 (KLVA_52160)	19445..19930	-	486	WP_119867226.1	TIR domain-containing protein	-
KI238_RS26930 (KLVA_52170)	19942..20469	-	528	WP_119867227.1	toll/interleukin-1 receptor domain-containing protein	-
KI238_RS26935 (KLVA_52180)	21023..21253	+	231	WP_119867228.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KI238_RS26940 (KLVA_52190)	21250..21666	+	417	WP_119867229.1	type II toxin-antitoxin system VapC family toxin	Toxin
KI238_RS26945 (KLVA_52210)	22170..23180	-	1011	WP_123797896.1	hypothetical protein	-
KI238_RS26950 (KLVA_52220)	23700..23930	+	231	WP_119867231.1	type II toxin-antitoxin system VapB family antitoxin	-
KI238_RS26955 (KLVA_52230)	23927..24343	+	417	WP_119867232.1	type II toxin-antitoxin system VapC family toxin	-
KI238_RS26960 (KLVA_52240)	24485..24703	+	219	WP_119867233.1	hypothetical protein	-
KI238_RS26965 (KLVA_52250)	24782..25108	+	327	WP_123797897.1	hypothetical protein	-
KI238_RS26970 (KLVA_52260)	25348..25605	+	258	WP_119867235.1	hypothetical protein	-
KI238_RS26975 (KLVA_52270)	25605..26171	+	567	WP_119867236.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..163252	163252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15082.51 Da
• Isoelectric Point: 7.8595

>T38900 WP_119867229.1 NZ_AP024593:21250-21666 [Klebsiella variicola]
VNKTYMLDTNICSFIMREQPEAVIKRLEQAVLRNQRIVISAITYAELRFGATGPKASPRHIALVDAFCARLDAVLPWDRA
AVDATTDIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLMLEDWVK

Nucleotide

• Length: 417 bp

>T38900 NZ_AP024593:21250-21666 [Klebsiella variicola]
GTGAACAAAACTTACATGCTCGATACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCGGTCATTAAGCGCCT
GGAGCAGGCGGTACTGCGCAATCAGCGTATCGTGATCTCCGCCATTACCTACGCGGAGCTGCGTTTCGGGGCCACCGGAC
CGAAGGCCTCACCGCGCCACATTGCGCTGGTTGACGCTTTCTGCGCCCGCCTCGATGCTGTCCTGCCTTGGGACCGCGCC
GCAGTGGACGCCACGACGGACATAAAAGTGGCGTTGCGGCTGGCCGGGACGCCTATTGGTCCGAATGACACGGCCATTGC
CGGGCACGCTATCGCCGCCGGCGCCATCCTAGTGACAAATAACACGCGGGAATTTGAACGGGTGCCGGGCCTGATGCTGG
AAGACTGGGTGAAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8682.75 Da
• Isoelectric Point: 4.4563

>AT38900 WP_119867228.1 NZ_AP024593:21023-21253 [Klebsiella variicola]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADADFMADREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT38900 NZ_AP024593:21023-21253 [Klebsiella variicola]
ATGCGCACAGTTTCAATTTTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGCGACCTGGATTTTGAGGGCGTCAG
TGAGCTGGAGATCGTCCGAGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAGGCCGACGCGGACTTTATGGCGGATCGCGAGGACGTTGTCAGCGATGAAGGACGATTTAATCTGTGA