Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5486230..5486855 Replicon chromosome
Accession NZ_AP024592
Organism Klebsiella variicola strain JCM 12419

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KI238_RS26480 Protein ID WP_008807903.1
Coordinates 5486230..5486613 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KI238_RS26485 Protein ID WP_004150355.1
Coordinates 5486613..5486855 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI238_RS26465 (KLVA_51290) 5483596..5484498 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KI238_RS26470 (KLVA_51300) 5484495..5485130 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KI238_RS26475 (KLVA_51310) 5485127..5486056 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KI238_RS26480 (KLVA_51320) 5486230..5486613 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KI238_RS26485 (KLVA_51330) 5486613..5486855 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KI238_RS26490 (KLVA_51340) 5487060..5487977 + 918 WP_008807904.1 alpha/beta hydrolase -
KI238_RS26495 (KLVA_51350) 5487992..5488933 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KI238_RS26500 (KLVA_51360) 5488978..5489415 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
KI238_RS26505 (KLVA_51370) 5489412..5490272 - 861 WP_008807907.1 virulence factor BrkB family protein -
KI238_RS26510 (KLVA_51380) 5490266..5490865 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T38899 WP_008807903.1 NZ_AP024592:c5486613-5486230 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T38899 NZ_AP024592:c5486613-5486230 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT38899 WP_004150355.1 NZ_AP024592:c5486855-5486613 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT38899 NZ_AP024592:c5486855-5486613 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References