Detailed information of TA system TA038897

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4930795..4931498
• Replicon: chromosome
• Accession: NZ_AP024592
• Organism: Klebsiella variicola strain JCM 12419

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KI238_RS23880
• Protein ID: WP_119867159.1
• Coordinates: 4930795..4931136 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: KI238_RS23885
• Protein ID: WP_119867160.1
• Coordinates: 4931172..4931498 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI238_RS23855 (KLVA_46360)	4927174..4928043	+	870	WP_087653704.1	HNH endonuclease	-
KI238_RS23860 (KLVA_46370)	4928324..4929121	-	798	WP_119867155.1	helix-turn-helix domain-containing protein	-
KI238_RS23865 (KLVA_46380)	4929243..4929518	-	276	WP_119867156.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KI238_RS23870 (KLVA_46390)	4929522..4929770	-	249	WP_119867157.1	ribbon-helix-helix domain-containing protein	-
KI238_RS23875 (KLVA_46400)	4929847..4930680	-	834	WP_119867158.1	DUF4942 domain-containing protein	-
KI238_RS23880 (KLVA_46410)	4930795..4931136	-	342	WP_119867159.1	TA system toxin CbtA family protein	Toxin
KI238_RS23885 (KLVA_46420)	4931172..4931498	-	327	WP_119867160.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KI238_RS23890 (KLVA_46430)	4931543..4932016	-	474	WP_119867161.1	DNA repair protein RadC	-
KI238_RS23895 (KLVA_46440)	4932087..4932908	-	822	WP_119867162.1	DUF932 domain-containing protein	-
KI238_RS23900 (KLVA_46450)	4933005..4933472	-	468	Protein_4704	hypothetical protein	-
KI238_RS23905 (KLVA_46460)	4933517..4934017	-	501	WP_227518038.1	hypothetical protein	-
KI238_RS23910 (KLVA_46470)	4934041..4934730	-	690	WP_119867163.1	WYL domain-containing protein	-
KI238_RS23915 (KLVA_46480)	4934943..4935818	-	876	WP_119867164.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4930795..4954047	23252

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12842.75 Da
• Isoelectric Point: 8.5195

>T38897 WP_119867159.1 NZ_AP024592:c4931136-4930795 [Klebsiella variicola]
MKTLPASTQRAAKPCPTPVVVWQTLLTRLLEQHYGLALNDTPFCDETVIQEHINAGITLADAVNFLVEKYELVRIDRRGF
SWQEQSPYLRAVDILRARQATGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T38897 NZ_AP024592:c4931136-4930795 [Klebsiella variicola]
ATGAAAACTTTACCTGCATCAACTCAGCGGGCGGCTAAGCCATGCCCGACGCCCGTAGTTGTCTGGCAAACGCTACTCAC
CCGTCTGCTGGAGCAACACTATGGTCTGGCTCTGAACGACACGCCATTCTGCGATGAAACAGTCATTCAGGAACATATTA
ACGCCGGTATCACACTGGCTGATGCCGTTAACTTTCTGGTGGAAAAGTACGAGCTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGATATTTTGAGGGCGAGACAGGCAACGGGTCTGTTACGACAAGG
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12348.91 Da
• Isoelectric Point: 5.9666

>AT38897 WP_119867160.1 NZ_AP024592:c4931498-4931172 [Klebsiella variicola]
MEKHDPTEWGLRRDITPRVGARLVQEGNQLHYLADRAGVTGTFNEAESRKLNAAFPHFVEQMELMLLSGELNPRYAHCVT
LYHDGFTCEADTLGSYGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT38897 NZ_AP024592:c4931498-4931172 [Klebsiella variicola]
ATGGAGAAACACGATCCCACCGAATGGGGGCTTCGACGCGATATCACGCCGCGTGTAGGAGCACGACTGGTACAGGAAGG
AAATCAACTGCATTACTTGGCTGACCGGGCCGGTGTCACCGGCACCTTTAATGAAGCTGAGAGCCGCAAACTGAATGCAG
CATTTCCGCATTTCGTGGAGCAGATGGAGTTAATGTTACTCTCCGGGGAACTAAACCCCCGTTATGCGCACTGTGTCACC
CTGTACCATGATGGATTTACCTGTGAAGCCGACACGCTTGGCTCTTATGGTTACGTCTACATCGTCATTTACCCTACTCA
ACGCTGA