Detailed information of TA system TA038878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 65258..65780
• Replicon: plasmid pCIAM-1
• Accession: NZ_AP024586
• Organism: Citrobacter amalonaticus strain JCM 1661

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: KI228_RS22600
• Protein ID: WP_141227313.1
• Coordinates: 65496..65780 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A376C2P3
• Locus tag: KI228_RS22595
• Protein ID: WP_048030981.1
• Coordinates: 65258..65506 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KI228_RS22575 (CIAM_43980)	61043..61948	+	906	WP_141227309.1	LysR family transcriptional regulator	-
KI228_RS22580 (CIAM_43990)	62074..62655	-	582	WP_141227310.1	NAD(P)H-dependent oxidoreductase	-
KI228_RS22585 (CIAM_44000)	62728..63918	-	1191	WP_141227311.1	MFS transporter	-
KI228_RS22590	64004..64798	+	795	WP_141227312.1	helix-turn-helix transcriptional regulator	-
KI228_RS22595 (CIAM_44020)	65258..65506	+	249	WP_048030981.1	plasmid stabilization protein	Antitoxin
KI228_RS22600 (CIAM_44030)	65496..65780	+	285	WP_141227313.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KI228_RS22605	65796..67138	-	1343	Protein_67	ISNCY family transposase	-
KI228_RS22610 (CIAM_44050)	67528..68799	-	1272	WP_141227314.1	Y-family DNA polymerase	-
KI228_RS22615 (CIAM_44060)	68799..69230	-	432	WP_040078803.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KI228_RS22620 (CIAM_44070)	69462..70433	+	972	WP_212807616.1	plasmid segregation protein ParM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..187150	187150

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11031.77 Da
• Isoelectric Point: 10.5388

>T38878 WP_141227313.1 NZ_AP024586:65496-65780 [Citrobacter amalonaticus]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNVTLNRN

Nucleotide

• Length: 285 bp

>T38878 NZ_AP024586:65496-65780 [Citrobacter amalonaticus]
ATGACCTATAAGTTGGCTTTCAACGAATCCGCTTTGAAGGAATGGAAGAAGCTGGGTCATACACTACAGGTGCAGTTTAA
AAAGAAGCTCAAAGAGCGACTGGAGAACCCCAGAGTTCCGGCATCACAGCTACATGGACGTAAGGACCAGTATAAAATTA
AGCTACGTGGTGCCGGCTACCGTCTTGTCTATCGTGTAGAGGACGACATTGTCACAGTCACTGTAATCGGCGTAGGCAAA
CGCGAAAACGATGACATCTATAATGTAACCCTGAATCGCAATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9039.25 Da
• Isoelectric Point: 3.9947

>AT38878 WP_048030981.1 NZ_AP024586:65258-65506 [Citrobacter amalonaticus]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGERVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT38878 NZ_AP024586:65258-65506 [Citrobacter amalonaticus]
ATGGCATTTCAGGTTCTTACCACTACTGCAGCCAGCATTACTGAGCTTAAACGCGACCCTATGGGTACTTTCCACGCCGG
GGATGGCGCTCCGGTAGCCATTCTTAATCGTAATGAGCCTGCATTCTACTGCGTGCCACCGGCACTATATGCCCAGTTGA
TGGAACTCCTGGAAGATGAAGAGCTGGGACGGATGGCTGATGAGCGAAGCGGGGAAAGGGTTATTGAGGTAAATATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A376C2P3