Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1294555..1295175 Replicon chromosome
Accession NZ_AP024585
Organism Citrobacter amalonaticus strain JCM 1661

Toxin (Protein)


Gene name Hha Uniprot ID V1H8E6
Locus tag KI228_RS06120 Protein ID WP_001280991.1
Coordinates 1294555..1294773 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A3Q8DH98
Locus tag KI228_RS06125 Protein ID WP_042324455.1
Coordinates 1294801..1295175 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KI228_RS06090 (CIAM_11910) 1290208..1290804 + 597 WP_061070461.1 membrane protein -
KI228_RS06095 (CIAM_11920) 1290887..1291240 + 354 WP_043001561.1 DUF1428 family protein -
KI228_RS06100 (CIAM_11930) 1291280..1292830 - 1551 WP_054176085.1 EAL domain-containing protein -
KI228_RS06105 1293053..1293193 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KI228_RS06110 (CIAM_11940) 1293243..1293713 - 471 WP_061070460.1 YlaC family protein -
KI228_RS06115 (CIAM_11950) 1293827..1294378 - 552 WP_044258934.1 maltose O-acetyltransferase -
KI228_RS06120 (CIAM_11960) 1294555..1294773 - 219 WP_001280991.1 HHA domain-containing protein Toxin
KI228_RS06125 (CIAM_11970) 1294801..1295175 - 375 WP_042324455.1 Hha toxicity modulator TomB Antitoxin
KI228_RS06130 (CIAM_11980) 1295687..1298836 - 3150 WP_043001557.1 efflux RND transporter permease AcrB -
KI228_RS06135 (CIAM_11990) 1298859..1300052 - 1194 WP_043001556.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8613.97 Da        Isoelectric Point: 8.9008

>T38865 WP_001280991.1 NZ_AP024585:c1294773-1294555 [Citrobacter amalonaticus]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T38865 NZ_AP024585:c1294773-1294555 [Citrobacter amalonaticus]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACAATTGACACACTTGAACGCGT
GATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCCGCCGATCACCGTCTTGCAGAAT
TGACGATGAATAAACTTTACGACAAGATCCCCTCCTCCGTCTGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8989

>AT38865 WP_042324455.1 NZ_AP024585:c1295175-1294801 [Citrobacter amalonaticus]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT38865 NZ_AP024585:c1295175-1294801 [Citrobacter amalonaticus]
ATGGATGAATACTCACCCAAAAGACATGATATAGCGCAGCTTAAATTTCTTTGTGAAACGCTTTATCACGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTTCAACTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAGGACAATAAGCTGATTGAGCAGATTGATGAATATCTG
GACGACACATTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTGCAGAAATGGCGGAAATCAGGTAATCGCTT
GTTCCGTTGCTTTGTGAATGCCACCAGAGCTAACCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4ICG

Antitoxin

Source ID Structure
AlphaFold DB A0A3Q8DH98

References