Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5311707..5312332 Replicon chromosome
Accession NZ_AP024579
Organism Klebsiella pneumoniae strain KpWEA4-2

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag L6443_RS25695 Protein ID WP_019705794.1
Coordinates 5311707..5312090 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6443_RS25700 Protein ID WP_004150355.1
Coordinates 5312090..5312332 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6443_RS25680 (MAKP6SUB2_49430) 5309073..5309975 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L6443_RS25685 (MAKP6SUB2_49440) 5309972..5310607 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6443_RS25690 (MAKP6SUB2_49450) 5310604..5311533 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6443_RS25695 (MAKP6SUB2_49460) 5311707..5312090 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
L6443_RS25700 (MAKP6SUB2_49470) 5312090..5312332 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6443_RS25705 (MAKP6SUB2_49480) 5312537..5313454 + 918 WP_012737090.1 alpha/beta hydrolase -
L6443_RS25710 (MAKP6SUB2_49490) 5313468..5314409 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
L6443_RS25715 (MAKP6SUB2_49500) 5314454..5314891 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6443_RS25720 (MAKP6SUB2_49510) 5314888..5315748 - 861 WP_004146232.1 virulence factor BrkB family protein -
L6443_RS25725 (MAKP6SUB2_49520) 5315742..5316341 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T38859 WP_019705794.1 NZ_AP024579:c5312090-5311707 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T38859 NZ_AP024579:c5312090-5311707 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT38859 WP_004150355.1 NZ_AP024579:c5312332-5312090 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT38859 NZ_AP024579:c5312332-5312090 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References