Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4103426..4104045 Replicon chromosome
Accession NZ_AP024579
Organism Klebsiella pneumoniae strain KpWEA4-2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L6443_RS19970 Protein ID WP_002892050.1
Coordinates 4103827..4104045 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L6443_RS19965 Protein ID WP_002892066.1
Coordinates 4103426..4103800 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6443_RS19955 (MAKP6SUB2_38450) 4098578..4099771 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6443_RS19960 (MAKP6SUB2_38460) 4099794..4102940 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L6443_RS19965 (MAKP6SUB2_38470) 4103426..4103800 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L6443_RS19970 (MAKP6SUB2_38480) 4103827..4104045 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L6443_RS19975 (MAKP6SUB2_38490) 4104204..4104770 + 567 WP_002892030.1 maltose O-acetyltransferase -
L6443_RS19980 4104742..4104882 - 141 WP_004147370.1 hypothetical protein -
L6443_RS19985 (MAKP6SUB2_38500) 4104903..4105373 + 471 WP_002892026.1 YlaC family protein -
L6443_RS19990 (MAKP6SUB2_38510) 4105348..4106799 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
L6443_RS19995 (MAKP6SUB2_38520) 4106900..4107598 + 699 WP_002892021.1 GNAT family protein -
L6443_RS20000 (MAKP6SUB2_38530) 4107595..4107735 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L6443_RS20005 (MAKP6SUB2_38540) 4107735..4107998 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38855 WP_002892050.1 NZ_AP024579:4103827-4104045 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T38855 NZ_AP024579:4103827-4104045 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT38855 WP_002892066.1 NZ_AP024579:4103426-4103800 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT38855 NZ_AP024579:4103426-4103800 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References