Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5213658..5214283 Replicon chromosome
Accession NZ_AP024576
Organism Klebsiella pneumoniae strain KpWEA4-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag L6441_RS25230 Protein ID WP_019705794.1
Coordinates 5213658..5214041 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6441_RS25235 Protein ID WP_004150355.1
Coordinates 5214041..5214283 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6441_RS25215 (MAKP6SUB1_48550) 5211024..5211926 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L6441_RS25220 (MAKP6SUB1_48560) 5211923..5212558 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6441_RS25225 (MAKP6SUB1_48570) 5212555..5213484 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6441_RS25230 (MAKP6SUB1_48580) 5213658..5214041 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
L6441_RS25235 (MAKP6SUB1_48590) 5214041..5214283 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6441_RS25240 (MAKP6SUB1_48600) 5214488..5215405 + 918 WP_012737090.1 alpha/beta hydrolase -
L6441_RS25245 (MAKP6SUB1_48610) 5215419..5216360 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
L6441_RS25250 (MAKP6SUB1_48620) 5216405..5216842 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6441_RS25255 (MAKP6SUB1_48630) 5216839..5217699 - 861 WP_004146232.1 virulence factor BrkB family protein -
L6441_RS25260 (MAKP6SUB1_48640) 5217693..5218292 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T38840 WP_019705794.1 NZ_AP024576:c5214041-5213658 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T38840 NZ_AP024576:c5214041-5213658 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT38840 WP_004150355.1 NZ_AP024576:c5214283-5214041 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT38840 NZ_AP024576:c5214283-5214041 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References