Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4005377..4005996 Replicon chromosome
Accession NZ_AP024576
Organism Klebsiella pneumoniae strain KpWEA4-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L6441_RS19505 Protein ID WP_002892050.1
Coordinates 4005778..4005996 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L6441_RS19500 Protein ID WP_002892066.1
Coordinates 4005377..4005751 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6441_RS19490 (MAKP6SUB1_37540) 4000529..4001722 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6441_RS19495 (MAKP6SUB1_37550) 4001745..4004891 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L6441_RS19500 (MAKP6SUB1_37560) 4005377..4005751 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L6441_RS19505 (MAKP6SUB1_37570) 4005778..4005996 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L6441_RS19510 (MAKP6SUB1_37580) 4006155..4006721 + 567 WP_002892030.1 maltose O-acetyltransferase -
L6441_RS19515 4006693..4006833 - 141 WP_004147370.1 hypothetical protein -
L6441_RS19520 (MAKP6SUB1_37590) 4006854..4007324 + 471 WP_002892026.1 YlaC family protein -
L6441_RS19525 (MAKP6SUB1_37600) 4007299..4008750 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
L6441_RS19530 (MAKP6SUB1_37610) 4008851..4009549 + 699 WP_002892021.1 GNAT family protein -
L6441_RS19535 (MAKP6SUB1_37620) 4009546..4009686 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L6441_RS19540 (MAKP6SUB1_37630) 4009686..4009949 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38836 WP_002892050.1 NZ_AP024576:4005778-4005996 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T38836 NZ_AP024576:4005778-4005996 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT38836 WP_002892066.1 NZ_AP024576:4005377-4005751 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT38836 NZ_AP024576:4005377-4005751 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References