Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5209291..5209916 Replicon chromosome
Accession NZ_AP024572
Organism Klebsiella pneumoniae strain KpWEA3

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag L6447_RS25205 Protein ID WP_019705794.1
Coordinates 5209291..5209674 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6447_RS25210 Protein ID WP_004150355.1
Coordinates 5209674..5209916 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6447_RS25190 (MAKP5_48470) 5206657..5207559 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L6447_RS25195 (MAKP5_48480) 5207556..5208191 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6447_RS25200 (MAKP5_48490) 5208188..5209117 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6447_RS25205 (MAKP5_48500) 5209291..5209674 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
L6447_RS25210 (MAKP5_48510) 5209674..5209916 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6447_RS25215 (MAKP5_48520) 5210121..5211038 + 918 WP_012737090.1 alpha/beta hydrolase -
L6447_RS25220 (MAKP5_48530) 5211052..5211993 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
L6447_RS25225 (MAKP5_48540) 5212038..5212475 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6447_RS25230 (MAKP5_48550) 5212472..5213332 - 861 WP_004146232.1 virulence factor BrkB family protein -
L6447_RS25235 (MAKP5_48560) 5213326..5213925 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T38821 WP_019705794.1 NZ_AP024572:c5209674-5209291 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T38821 NZ_AP024572:c5209674-5209291 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT38821 WP_004150355.1 NZ_AP024572:c5209916-5209674 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT38821 NZ_AP024572:c5209916-5209674 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References