Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4727423..4727939 Replicon chromosome
Accession NZ_AP024570
Organism Klebsiella pneumoniae strain KpWEA2

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag L6448_RS22885 Protein ID WP_004178374.1
Coordinates 4727423..4727707 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag L6448_RS22890 Protein ID WP_002886901.1
Coordinates 4727697..4727939 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6448_RS22860 (MAKP4_44140) 4722819..4723082 - 264 WP_004152271.1 PTS sugar transporter subunit IIB -
L6448_RS22865 4723212..4723385 + 174 WP_004222159.1 hypothetical protein -
L6448_RS22870 (MAKP4_44150) 4723388..4724131 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
L6448_RS22875 (MAKP4_44160) 4724488..4726626 + 2139 WP_032415880.1 anaerobic ribonucleoside-triphosphate reductase -
L6448_RS22880 (MAKP4_44170) 4726955..4727419 + 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
L6448_RS22885 (MAKP4_44180) 4727423..4727707 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6448_RS22890 (MAKP4_44190) 4727697..4727939 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
L6448_RS22895 (MAKP4_44200) 4728017..4729927 - 1911 WP_009486549.1 PRD domain-containing protein -
L6448_RS22900 (MAKP4_44210) 4729950..4731104 - 1155 WP_032415878.1 lactonase family protein -
L6448_RS22905 (MAKP4_44220) 4731171..4731911 - 741 WP_004186692.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T38800 WP_004178374.1 NZ_AP024570:c4727707-4727423 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T38800 NZ_AP024570:c4727707-4727423 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTTAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT38800 WP_002886901.1 NZ_AP024570:c4727939-4727697 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT38800 NZ_AP024570:c4727939-4727697 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References