Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4001011..4001630 Replicon chromosome
Accession NZ_AP024570
Organism Klebsiella pneumoniae strain KpWEA2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L6448_RS19470 Protein ID WP_002892050.1
Coordinates 4001412..4001630 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L6448_RS19465 Protein ID WP_002892066.1
Coordinates 4001011..4001385 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6448_RS19455 (MAKP4_37510) 3996163..3997356 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6448_RS19460 (MAKP4_37520) 3997379..4000525 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L6448_RS19465 (MAKP4_37530) 4001011..4001385 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L6448_RS19470 (MAKP4_37540) 4001412..4001630 + 219 WP_002892050.1 HHA domain-containing protein Toxin
L6448_RS19475 (MAKP4_37550) 4001789..4002355 + 567 WP_002892030.1 maltose O-acetyltransferase -
L6448_RS19480 4002327..4002467 - 141 WP_004147370.1 hypothetical protein -
L6448_RS19485 (MAKP4_37560) 4002488..4002958 + 471 WP_002892026.1 YlaC family protein -
L6448_RS19490 (MAKP4_37570) 4002933..4004384 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
L6448_RS19495 (MAKP4_37580) 4004485..4005183 + 699 WP_002892021.1 GNAT family protein -
L6448_RS19500 (MAKP4_37590) 4005180..4005320 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L6448_RS19505 (MAKP4_37600) 4005320..4005583 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38798 WP_002892050.1 NZ_AP024570:4001412-4001630 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T38798 NZ_AP024570:4001412-4001630 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT38798 WP_002892066.1 NZ_AP024570:4001011-4001385 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT38798 NZ_AP024570:4001011-4001385 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References