Detailed information of TA system TA038766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 205600..206339
• Replicon: chromosome
• Accession: NZ_AP024568
• Organism: Klebsiella pneumoniae strain KpWEA1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: L6449_RS01010
• Protein ID: WP_032415800.1
• Coordinates: 205854..206339 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: L6449_RS01005
• Protein ID: WP_003026799.1
• Coordinates: 205600..205866 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6449_RS00990 (MAKP3_01920)	201103..203172	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
L6449_RS00995	203469..204838	+	1370	WP_087830479.1	IS3 family transposase	-
L6449_RS01000 (MAKP3_01960)	205039..205467	+	429	WP_004901287.1	GFA family protein	-
L6449_RS01005 (MAKP3_01970)	205600..205866	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
L6449_RS01010	205854..206339	+	486	WP_032415800.1	GNAT family N-acetyltransferase	Toxin
L6449_RS01015 (MAKP3_01980)	206683..206835	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
L6449_RS01020 (MAKP3_01990)	207137..208756	+	1620	WP_032415799.1	ABC-F family ATP-binding cassette domain-containing protein	-
L6449_RS01025 (MAKP3_02000)	208855..209067	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
L6449_RS01030 (MAKP3_02010)	209320..209610	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
L6449_RS01035 (MAKP3_02020)	209856..211211	-	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	204113..204838	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17624.46 Da
• Isoelectric Point: 10.1428

>T38766 WP_032415800.1 NZ_AP024568:205854-206339 [Klebsiella pneumoniae]
VGRITAPEPLCSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T38766 NZ_AP024568:205854-206339 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATGCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT38766 WP_003026799.1 NZ_AP024568:205600-205866 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT38766 NZ_AP024568:205600-205866 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66