Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 3744144..3744715 Replicon chromosome
Accession NZ_AP024565
Organism Microcystis aeruginosa strain NIES-88

Toxin (Protein)


Gene name vapC Uniprot ID I4IBX6
Locus tag LC603172_RS18770 Protein ID WP_002759489.1
Coordinates 3744374..3744715 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1V4BQA4
Locus tag LC603172_RS18765 Protein ID WP_039899814.1
Coordinates 3744144..3744377 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LC603172_RS18740 (MAN88_36430) 3739596..3740444 + 849 WP_002760038.1 IS630-like element ISMae21 family transposase -
LC603172_RS18745 (MAN88_36440) 3740698..3742323 - 1626 WP_002804201.1 chaperonin GroEL -
LC603172_RS18750 (MAN88_36450) 3742370..3742681 - 312 WP_002737272.1 co-chaperone GroES -
LC603172_RS18755 (MAN88_36460) 3743016..3743207 + 192 WP_072319587.1 hypothetical protein -
LC603172_RS18760 (MAN88_36470) 3743390..3743818 - 429 WP_002744522.1 CAAD domain-containing protein -
LC603172_RS18765 (MAN88_36480) 3744144..3744377 + 234 WP_039899814.1 DUF433 domain-containing protein Antitoxin
LC603172_RS18770 (MAN88_36490) 3744374..3744715 + 342 WP_002759489.1 DUF5615 family PIN-like protein Toxin
LC603172_RS18775 (MAN88_36500) 3744725..3747046 - 2322 WP_061430764.1 DNA helicase PcrA -
LC603172_RS18780 (MAN88_36510) 3747372..3748622 + 1251 WP_012267167.1 methionine adenosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3739596..3740444 848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(1-78)

Antitoxin

Protein of unknown function DUF433

(11-66)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12508.41 Da        Isoelectric Point: 4.2544

>T38763 WP_002759489.1 NZ_AP024565:3744374-3744715 [Microcystis aeruginosa]
MKIWIDAQLPPTLASWLSATFDLEASSLRDLSLRDAQDIEIFAAARAENAVIMTKDSDFIDLVCRLGTPPQILWLTCGNV
TNQNLRRLLTATLPDALKQLEQGTIIVEISNTP

Download         Length: 342 bp

>T38763 NZ_AP024565:3744374-3744715 [Microcystis aeruginosa]
ATGAAAATTTGGATTGATGCTCAACTACCTCCAACTCTAGCAAGCTGGTTATCAGCGACCTTTGATTTAGAAGCATCGTC
CCTGCGAGACTTATCCCTTCGAGATGCTCAAGATATCGAGATCTTTGCAGCAGCGCGAGCGGAAAATGCCGTGATTATGA
CCAAAGATAGTGACTTTATTGATTTGGTTTGTCGTCTGGGAACACCTCCTCAAATTTTATGGTTAACCTGTGGTAATGTC
ACAAATCAGAATCTGCGGCGATTACTGACAGCTACTTTACCCGATGCGTTGAAACAACTAGAGCAAGGAACAATAATTGT
AGAGATTAGCAATACTCCTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8499.91 Da        Isoelectric Point: 4.2677

>AT38763 WP_039899814.1 NZ_AP024565:3744144-3744377 [Microcystis aeruginosa]
MDSTVSLLTRITQTPGQCGGRPCIRGMRIRVTDILEMLAENVSITEILEDFPDLELADIQACLLFAARCTDFPKLTA

Download         Length: 234 bp

>AT38763 NZ_AP024565:3744144-3744377 [Microcystis aeruginosa]
ATGGACTCAACAGTTAGCTTGCTTACCCGCATTACTCAAACACCCGGCCAGTGTGGTGGTCGTCCTTGTATTCGGGGGAT
GCGAATTCGAGTAACGGATATTTTGGAAATGTTAGCGGAGAATGTGAGCATAACTGAAATTCTAGAAGACTTTCCCGATT
TGGAACTCGCAGATATCCAAGCTTGTTTACTTTTTGCAGCACGGTGTACGGATTTTCCTAAACTAACGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I4IBX6

Antitoxin

Source ID Structure
AlphaFold DB A0A1V4BQA4

References