Detailed information of TA system TA038761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-UPF0150
• Location: 3324709..3325142
• Replicon: chromosome
• Accession: NZ_AP024565
• Organism: Microcystis aeruginosa strain NIES-88

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L7ED23
• Locus tag: LC603172_RS16420
• Protein ID: WP_002733933.1
• Coordinates: 3324709..3324933 (-)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L7EDS5
• Locus tag: LC603172_RS16425
• Protein ID: WP_002734412.1
• Coordinates: 3324930..3325142 (-)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC603172_RS16390 (MAN88_31890)	3320217..3321593	-	1377	WP_239697977.1	hypothetical protein	-
LC603172_RS16395 (MAN88_31900)	3321996..3323432	+	1437	WP_061430491.1	folate/biopterin family MFS transporter	-
LC603172_RS16400 (MAN88_31910)	3323529..3323750	-	222	WP_002732388.1	type II toxin-antitoxin system HicA family toxin	-
LC603172_RS16405 (MAN88_31920)	3323750..3323995	-	246	WP_002753457.1	type II toxin-antitoxin system HicB family antitoxin	-
LC603172_RS16410 (MAN88_31930)	3324101..3324289	-	189	WP_039899746.1	hypothetical protein	-
LC603172_RS16415 (MAN88_31940)	3324359..3324631	-	273	WP_002773681.1	hypothetical protein	-
LC603172_RS16420 (MAN88_31950)	3324709..3324933	-	225	WP_002733933.1	type II toxin-antitoxin system HicA family toxin	Toxin
LC603172_RS16425 (MAN88_31960)	3324930..3325142	-	213	WP_002734412.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LC603172_RS16430 (MAN88_31970)	3325521..3325820	+	300	WP_002763657.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LC603172_RS16435 (MAN88_31980)	3325804..3326076	+	273	WP_002763656.1	Txe/YoeB family addiction module toxin	-
LC603172_RS16440 (MAN88_31990)	3326156..3326881	-	726	WP_061430492.1	hypothetical protein	-
LC603172_RS16445 (MAN88_32000)	3327295..3329466	+	2172	WP_061430494.1	glutamine synthetase III	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (9-65)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-62)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8395.90 Da
• Isoelectric Point: 11.2578

>T38761 WP_002733933.1 NZ_AP024565:c3324933-3324709 [Microcystis aeruginosa]
MSELPPIAGRQVVKALGKIGYELDRQRGSHIILRQKAYPYRRITIPDHREVAKGTLRSIIRQAGLTVAEFKTLL

Nucleotide

• Length: 225 bp

>T38761 NZ_AP024565:c3324933-3324709 [Microcystis aeruginosa]
ATGAGTGAACTCCCTCCTATCGCTGGTCGCCAAGTCGTTAAAGCACTGGGCAAGATTGGCTACGAATTAGATCGGCAGCG
AGGAAGTCATATCATACTCAGACAAAAAGCCTATCCCTATCGACGCATCACCATCCCTGACCATCGAGAAGTGGCTAAGG
GAACGCTGAGATCTATCATTCGTCAAGCGGGATTGACAGTGGCAGAGTTTAAAACTCTCCTTTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7818.89 Da
• Isoelectric Point: 3.8618

>AT38761 WP_002734412.1 NZ_AP024565:c3325142-3324930 [Microcystis aeruginosa]
MKYRVLIEQDEDGIYVAEVPSLPGCISQGNTREELLANIQEAIALYLESLEAHNEPIPPPISEELVEVKL

Nucleotide

• Length: 213 bp

>AT38761 NZ_AP024565:c3325142-3324930 [Microcystis aeruginosa]
ATGAAATATCGCGTCTTAATTGAGCAAGATGAAGATGGCATTTATGTGGCTGAAGTTCCCTCCCTACCCGGCTGTATTTC
TCAAGGCAATACCCGGGAAGAGTTACTCGCCAACATTCAGGAAGCAATTGCTTTATATCTAGAAAGCCTAGAAGCGCACA
ATGAACCGATTCCACCACCAATTTCTGAGGAACTGGTAGAGGTTAAATTATGA

Structures

Toxin

• AlphaFold DB: L7ED23

Antitoxin

• AlphaFold DB: L7EDS5