Detailed information of TA system TA038757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1887703..1888274
• Replicon: chromosome
• Accession: NZ_AP024565
• Organism: Microcystis aeruginosa strain NIES-88

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LC603172_RS09250
• Protein ID: WP_061431141.1
• Coordinates: 1887942..1888274 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I4GAK8
• Locus tag: LC603172_RS09245
• Protein ID: WP_002772118.1
• Coordinates: 1887703..1887945 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC603172_RS09225 (MAN88_17980)	1883933..1884394	-	462	WP_002780536.1	hypothetical protein	-
LC603172_RS09230 (MAN88_17990)	1884750..1885727	-	978	WP_061431144.1	helix-turn-helix domain-containing protein	-
LC603172_RS09235 (MAN88_18000)	1886140..1886802	+	663	WP_061431143.1	potassium channel family protein	-
LC603172_RS09240 (MAN88_18010)	1886835..1887611	+	777	WP_061431142.1	DUF2092 domain-containing protein	-
LC603172_RS09245 (MAN88_18020)	1887703..1887945	+	243	WP_002772118.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LC603172_RS09250	1887942..1888274	+	333	WP_061431141.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LC603172_RS09255 (MAN88_18040)	1888457..1889350	+	894	WP_061431140.1	DUF6515 family protein	-
LC603172_RS09260 (MAN88_18050)	1889347..1892409	-	3063	WP_061431139.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-108)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12576.68 Da
• Isoelectric Point: 10.1581

>T38757 WP_061431141.1 NZ_AP024565:1887942-1888274 [Microcystis aeruginosa]
VTYIPERGDFLRLSFDPQIGHEQMGNRPALVVSPIDFNRKIGFAFVCPVSNTQRQNPFYVKIPDGEAVTGVIMVDQLRSL
DFRARKASFIGKCPERLLQDVLRRIKPILF

Nucleotide

• Length: 333 bp

>T38757 NZ_AP024565:1887942-1888274 [Microcystis aeruginosa]
GTGACATACATTCCAGAACGTGGAGATTTCCTGCGGTTGAGCTTCGACCCACAAATTGGGCATGAGCAAATGGGTAATCG
TCCAGCACTGGTGGTAAGCCCTATTGATTTTAATCGCAAGATTGGTTTTGCCTTTGTCTGTCCGGTGAGCAATACGCAAC
GCCAGAACCCTTTCTACGTCAAGATTCCAGATGGAGAGGCAGTGACAGGAGTTATCATGGTAGATCAGCTGCGATCGTTG
GACTTCCGAGCGAGAAAAGCGAGCTTTATCGGCAAGTGTCCAGAACGGTTGCTCCAGGATGTTCTCAGAAGAATTAAGCC
GATTCTGTTCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8942.23 Da
• Isoelectric Point: 3.9345

>AT38757 WP_002772118.1 NZ_AP024565:1887703-1887945 [Microcystis aeruginosa]
MKSQIGRWGNSLAIRIPKYAVEALNLNPNDVVECSVENGKLVIELIQALPELSLEELLAEVVESPEPEVDWGRPMGEEVW

Nucleotide

• Length: 243 bp

>AT38757 NZ_AP024565:1887703-1887945 [Microcystis aeruginosa]
ATGAAGTCGCAGATTGGACGCTGGGGTAATTCTTTAGCGATTCGGATTCCCAAGTATGCGGTGGAAGCACTAAACCTCAA
TCCCAATGACGTTGTTGAATGTTCTGTGGAAAACGGAAAGCTAGTAATTGAGCTAATTCAAGCCTTGCCTGAGTTAAGCC
TAGAAGAACTACTGGCTGAGGTTGTTGAATCACCGGAGCCGGAAGTGGATTGGGGACGGCCAATGGGAGAGGAGGTTTGG
TGA

Structures

Antitoxin

• AlphaFold DB: I4GAK8