Detailed information of TA system TA038756

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1725044..1725903
• Replicon: chromosome
• Accession: NZ_AP024565
• Organism: Microcystis aeruginosa strain NIES-88

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: B0JKU9
• Locus tag: LC603172_RS08470
• Protein ID: WP_012266042.1
• Coordinates: 1725505..1725903 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: LC603172_RS08465
• Protein ID: WP_061431221.1
• Coordinates: 1725044..1725463 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LC603172_RS08440	1720408..1720569	-	162	Protein_1687	helix-turn-helix domain-containing protein	-
LC603172_RS08445 (MAN88_16340)	1720672..1721553	-	882	WP_061431223.1	WYL domain-containing protein	-
LC603172_RS08450 (MAN88_16350)	1721653..1723818	+	2166	WP_061431222.1	type I-D CRISPR-associated helicase Cas3'	-
LC603172_RS08455 (MAN88_16360)	1723865..1724098	+	234	WP_002778649.1	hypothetical protein	-
LC603172_RS08460 (MAN88_16370)	1724484..1724798	+	315	WP_002778651.1	hypothetical protein	-
LC603172_RS08465 (MAN88_16380)	1725044..1725463	+	420	WP_061431221.1	nucleotidyltransferase domain-containing protein	Antitoxin
LC603172_RS08470 (MAN88_16390)	1725505..1725903	+	399	WP_012266042.1	DUF86 domain-containing protein	Toxin
LC603172_RS08475 (MAN88_16400)	1725896..1726354	+	459	WP_061431220.1	nucleotidyltransferase domain-containing protein	-
LC603172_RS08480 (MAN88_16410)	1726358..1726792	+	435	WP_002778659.1	DUF86 domain-containing protein	-
LC603172_RS08485 (MAN88_16420)	1726785..1730228	+	3444	WP_221275507.1	type I-D CRISPR-associated protein Cas10d/Csc3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (1-117)

Antitoxin

• Polymerase beta, nucleotidyltransferase (6-101)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15386.66 Da
• Isoelectric Point: 4.6345

>T38756 WP_012266042.1 NZ_AP024565:1725505-1725903 [Microcystis aeruginosa]
MNQYLAELSEYGSITLEDYRTLRERQLAIERLIQLIVQTGIDINYQILKCLDIESPNNARDALFQIVELGILEEHLAVQL
AESIKLRNLLVHLYKKIDPDIVHSSIANILRDYPRYQRSIVQYLDFLEAENG

Nucleotide

• Length: 399 bp

>T38756 NZ_AP024565:1725505-1725903 [Microcystis aeruginosa]
ATGAATCAATACCTCGCCGAACTTAGCGAGTACGGTTCGATCACCTTAGAGGATTATCGTACATTAAGAGAACGTCAATT
AGCCATAGAAAGGTTAATTCAGCTTATCGTTCAAACTGGCATCGATATCAATTACCAGATTTTAAAATGTTTAGATATCG
AGTCTCCGAATAATGCTCGCGACGCATTATTTCAAATCGTAGAACTGGGAATTTTAGAAGAACACCTAGCCGTTCAACTG
GCCGAGTCTATTAAATTAAGAAACTTACTCGTACACCTATACAAGAAAATTGATCCCGATATCGTTCATTCCTCGATCGC
TAATATTTTACGAGATTATCCCCGCTACCAACGCTCGATCGTTCAATATCTAGATTTCTTGGAGGCAGAAAATGGTTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 16164.68 Da
• Isoelectric Point: 5.0863

>AT38756 WP_061431221.1 NZ_AP024565:1725044-1725463 [Microcystis aeruginosa]
MNRLVEKITAIVEKLPNLKLLILFGSRARGEHKPDSDWDFAVLYEERSDQKDISSLLKIYTLLEQALEIPEDKIDVIDLK
GCSPILAHYVARDGQLLYERETGLFEVFKEKFLMNPEESKALYRQLRNNLEISLREKGV

Nucleotide

• Length: 420 bp

>AT38756 NZ_AP024565:1725044-1725463 [Microcystis aeruginosa]
ATGAATAGGCTAGTAGAAAAAATTACGGCTATTGTCGAAAAACTACCGAATTTAAAACTATTAATTCTCTTCGGTTCTAG
AGCGCGTGGAGAGCATAAACCTGATAGCGATTGGGATTTCGCTGTTTTATACGAGGAAAGAAGCGATCAAAAGGATATTT
CGAGTCTATTGAAAATTTATACACTACTCGAACAAGCTTTAGAGATTCCCGAGGACAAAATTGATGTGATTGATTTAAAA
GGATGTTCGCCGATCCTCGCTCACTATGTCGCGCGGGATGGTCAACTTCTATACGAACGGGAAACGGGACTATTCGAGGT
ATTTAAAGAAAAATTTCTCATGAATCCTGAAGAATCGAAAGCGTTATACCGTCAATTACGGAATAATCTGGAGATTTCTC
TACGAGAAAAAGGAGTGTAA

Structures

Toxin

• AlphaFold DB: B0JKU9