Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3544470..3545088 Replicon chromosome
Accession NZ_AP024561
Organism Escherichia coli strain NIPH17_0036

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag FLY99_RS17100 Protein ID WP_001291435.1
Coordinates 3544470..3544688 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag FLY99_RS17105 Protein ID WP_000344800.1
Coordinates 3544714..3545088 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY99_RS17065 (3539759) 3539759..3540331 + 573 WP_000779831.1 YbaY family lipoprotein -
FLY99_RS17070 (3540362) 3540362..3540673 - 312 WP_000409911.1 MGMT family protein -
FLY99_RS17080 (3541052) 3541052..3541405 + 354 WP_000878140.1 DUF1428 domain-containing protein -
FLY99_RS17085 (3541447) 3541447..3542997 - 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
FLY99_RS17090 (3543161) 3543161..3543631 - 471 WP_000136192.1 YlaC family protein -
FLY99_RS17095 (3543747) 3543747..3544298 - 552 WP_000102568.1 maltose O-acetyltransferase -
FLY99_RS17100 (3544470) 3544470..3544688 - 219 WP_001291435.1 HHA domain-containing protein Toxin
FLY99_RS17105 (3544714) 3544714..3545088 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
FLY99_RS17110 (3545634) 3545634..3548783 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
FLY99_RS17115 (3548806) 3548806..3549999 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T38745 WP_001291435.1 NZ_AP024561:c3544688-3544470 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T38745 NZ_AP024561:c3544688-3544470 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT38745 WP_000344800.1 NZ_AP024561:c3545088-3544714 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT38745 NZ_AP024561:c3545088-3544714 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References