Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2786821..2787318 Replicon chromosome
Accession NZ_AP024561
Organism Escherichia coli strain NIPH17_0036

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag FLY99_RS13450 Protein ID WP_001173343.1
Coordinates 2787145..2787318 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag FLY99_RS13445 Protein ID WP_001425728.1
Coordinates 2786821..2787117 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY99_RS13425 (2783552) 2783552..2784271 - 720 WP_000611288.1 two-component system response regulator DcuR -
FLY99_RS13430 (2784268) 2784268..2785899 - 1632 WP_001216487.1 sensor histidine kinase -
FLY99_RS13435 (2786080) 2786080..2786310 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
FLY99_RS13440 (2786322) 2786322..2786594 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
FLY99_RS13445 (2786821) 2786821..2787117 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
FLY99_RS13450 (2787145) 2787145..2787318 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
FLY99_RS13455 (2787437) 2787437..2788954 - 1518 WP_001295074.1 lysine--tRNA ligase -
FLY99_RS13460 (2789191) 2789191..2790648 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T38744 WP_001173343.1 NZ_AP024561:2787145-2787318 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T38744 NZ_AP024561:2787145-2787318 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT38744 WP_001425728.1 NZ_AP024561:2786821-2787117 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT38744 NZ_AP024561:2786821-2787117 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References