Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1961693..1962279 Replicon chromosome
Accession NZ_AP024561
Organism Escherichia coli strain NIPH17_0036

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag FLY99_RS09605 Protein ID WP_042014162.1
Coordinates 1962049..1962279 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag FLY99_RS09600 Protein ID WP_000593555.1
Coordinates 1961693..1962052 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY99_RS09575 (1957825) 1957825..1958769 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
FLY99_RS09580 (1958766) 1958766..1959599 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
FLY99_RS09585 (1959599) 1959599..1960363 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
FLY99_RS09590 (1960360) 1960360..1961166 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
FLY99_RS09595 (1961172) 1961172..1961573 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
FLY99_RS09600 (1961693) 1961693..1962052 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
FLY99_RS09605 (1962049) 1962049..1962279 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
FLY99_RS09610 (1962383) 1962383..1963507 - 1125 WP_001314210.1 ABC transporter permease -
FLY99_RS09615 (1963507) 1963507..1966242 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T38742 WP_042014162.1 NZ_AP024561:c1962279-1962049 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T38742 NZ_AP024561:c1962279-1962049 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT38742 WP_000593555.1 NZ_AP024561:c1962052-1961693 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT38742 NZ_AP024561:c1962052-1961693 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References