Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1312693..1313347 Replicon chromosome
Accession NZ_AP024561
Organism Escherichia coli strain NIPH17_0036

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag FLY99_RS06300 Protein ID WP_000244777.1
Coordinates 1312693..1313100 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag FLY99_RS06305 Protein ID WP_000354046.1
Coordinates 1313081..1313347 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FLY99_RS06280 (1308650) 1308650..1310383 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
FLY99_RS06285 (1310389) 1310389..1311099 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
FLY99_RS06290 (1311124) 1311124..1312020 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
FLY99_RS06295 (1312132) 1312132..1312653 + 522 WP_001055874.1 flavodoxin FldB -
FLY99_RS06300 (1312693) 1312693..1313100 - 408 WP_000244777.1 protein YgfX Toxin
FLY99_RS06305 (1313081) 1313081..1313347 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
FLY99_RS06310 (1313590) 1313590..1314570 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
FLY99_RS06315 (1314647) 1314647..1315306 - 660 WP_000250274.1 hemolysin III family protein -
FLY99_RS06320 (1315470) 1315470..1315781 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
FLY99_RS06325 (1315826) 1315826..1317259 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
FLY99_RS06330 (1317316) 1317316..1318059 - 744 Protein_1237 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T38737 WP_000244777.1 NZ_AP024561:c1313100-1312693 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T38737 NZ_AP024561:c1313100-1312693 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT38737 WP_000354046.1 NZ_AP024561:c1313347-1313081 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT38737 NZ_AP024561:c1313347-1313081 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References