Detailed information of TA system TA038734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 872201..872826
• Replicon: chromosome
• Accession: NZ_AP024561
• Organism: Escherichia coli strain NIPH17_0036

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FLY99_RS04195
• Protein ID: WP_000911330.1
• Coordinates: 872201..872599 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: FLY99_RS04200
• Protein ID: WP_000450524.1
• Coordinates: 872599..872826 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FLY99_RS04175 (867918)	867918..868664	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
FLY99_RS04180 (868746)	868746..869087	-	342	Protein_820	esterase	-
FLY99_RS04185 (869161)	869161..871176	-	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
FLY99_RS04190 (871191)	871191..872054	-	864	WP_001267507.1	neutral zinc metallopeptidase	-
FLY99_RS04195 (872201)	872201..872599	-	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
FLY99_RS04200 (872599)	872599..872826	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
FLY99_RS04205 (872980)	872980..873693	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
FLY99_RS04210 (873906)	873906..874940	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
FLY99_RS04215 (874957)	874957..875835	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
FLY99_RS04220 (875981)	875981..876553	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
FLY99_RS04225 (876553)	876553..877023	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T38734 WP_000911330.1 NZ_AP024561:c872599-872201 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T38734 NZ_AP024561:c872599-872201 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT38734 WP_000450524.1 NZ_AP024561:c872826-872599 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT38734 NZ_AP024561:c872826-872599 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33